Steve achieved worldwide fame from the television program The Crocodile Hunter, an internationally broadcast wildlife documentary series co-hosted with his wife Terri Irwin. Together, they also co-owned and operated Australia Zoo, founded by his parents in Beerwah, Queensland. He died in 2006 after being fatally pierced in the chest by a stingray barb while filming in Australia's Great Barrier Reef.

On 4 September 2006, Irwin was fatally pierced in the chest by a stingray spine while snorkeling at the Great Barrier Reef, at Batt Reef, which is located off the coast of Port Douglas in north Queensland. Irwin was in the area filming his own documentary, Ocean's Deadliest, but weather had stalled filming. Irwin decided to take the opportunity to film some shallow water shots for a segment in the television program his daughter Bindi Irwin was hosting,[57] when, according to his friend and colleague, John Stainton, he swam too close to one of the stingrays. "He came on top of the stingray and the stingray's barb went up and into his chest and put a hole into his heart," said Stainton, who was on board Irwin's boat the Croc One.